By Timothy Richard Parsons
An advent to the quantitative research of seawater, describing intimately organic and chemical innovations, that are thought of to be among these typically utilized by organic oceanographers. The guide offers entire directions for the addition of reagents and calculation of effects with regard fabric for every strategy in order that the unique texts should be consulted if beneficial. as a rule, the concepts require not less than past expert education and strategies desiring very pricey gear were kept away from
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Additional info for A Manual of Chemical & Biological Methods for Seawater Analysis
The assay of other growth factors follows a very similar procedure to that described below if the medium is modified to be limiting to the substance for which the analysis is required and vitamin B i 2 is added in excess. Examples of such analyses are given for biotin (Carlucci and Silbernagel, Can. J. , 13: 979, 1967) and for thiamine (Carlucci and Silbernagel, Can. J. , 12: 1079, 1966). The choice of organisms used in such assays depends upon a demonstrated requirement for the chemical species being assayed.
Soluble Organic Material 43 3. Elute with 80 ml of reagent grade 2N N H 4O H into a rotary evaporator flask and reduce the volume under vacuum at 40°C. 4. Transfer the concentrate (ca. 10 ml) into a 25-ml capacity rotary evaporator flask and evaporate to dryness at 40°C. 5. 02N N a O H into the flask in order to remove ammonia and evaporate to dryness again at 40°C. 6. 02N HC1. 7. Measure the fluorescence of the sample according to Section F - a , 1-3. G. Determination of blank Determination of the blank for direct measurement of the sample (Section F - a ) is carried out using redistilled water instead of a seawater sample, employing the procedure described in Section F - a , 1-3.
2, Section D . E. Special reagents 1. Hydrochloric acid Reagent grade concentrated hydrochloric acid (sp. 19) is used in this experiment. 2. 8 g of reagent grade sodium hydroxide in redistilled water and make up the volume to 1 1. 3. 2, Section E, with respect to the other reagents. 46 Soluble Organic F. Experimental Material procedure 1. Pipette 5 ml of filtered water into a glass ampule and add the concentrated HC1 to make the final acidity 6 Normal. 2. Aerate the sample in the tube with highly pure nitrogen gas for 5 min and seal the tube (Note a).
A Manual of Chemical & Biological Methods for Seawater Analysis by Timothy Richard Parsons